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To investigate nutritive values of a feed fermented with basidiomycetes, among the isolated strains, Lyophyllum decastes (Fr.) Sing. was found with the greatest enzyme productivity and rapid mycelial growth in rice straw medium. Optimum temperature, pH and moisture content for mycelial growth arid enzyme production of the strain were 25∼30℃, pH 4.0∼7.0 and 70∼75%, respectively. Fifteen days of culture were required for the highest enzyme productivity. Among the sub-materials added, 30∼40% of rice bran and 10∼20% of defatted perilla seeds were effective for the enzyme production, but caused a reduced mycelial growth. The greatest effect of an addition of inorganic salts was obtained with 0.36∼0.72 % of (NH₄)₂HPO₄. When 40 mesh or smaller rice straw and steam treatment at 0.5 ㎏/㎠ were used, the mycelial growth decreased, whereas the enzyme production increased. The mycelial growth and enzyme production increased when Ca(OH)₂ was used as the alkali treatment, but decreased with increasing concentration of NaOH. As the fermentation proceeded, the amounts of ash, reducing sugar and total nitrogen increased, but cellulose, lignin and pentosan decreased. When the rice straw was treated with alkali, the amounts of ash, total nitrogen and lignin decreased, but reducing sugar and cellylose increased. At higher NaOH concentration, the variation become greater. The in vitro dry matter digestibility of the products increased from 55.03% at the beginning of the fermentation to 62.72% at 45 days after fermentation. The most effective alkali treatment on the digestibility of rice straw was KOH followed by NaOH. However, the digestibility increased with increasing concentration of NaOH. The digestibility of pretreated with alkali increased after fermentation as well.
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